Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat S100 Calcium Binding Protein A9/Calgranulin B (also known as S100A9) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat S100A9 antibody. S100A9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat S100A9 Antibody is added and binds to S100A9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated S100A9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat S100A9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm